灯盏花素对缺血再灌注大鼠心肌超微结构及细胞凋亡的影响

Influence of Breviscapine on Ultrastructure and Apoptosis of Myocardial Cellsafter Myocardial Ischemia and Eperfusion in Rat

摘要:

目的 :研究灯盏花素(breviscapine)对在体大鼠心肌缺血再灌注损伤的保护作用及其机制。 方法 :40只SD大鼠随机分为假手术组,缺血再灌注组(IR组),灯盏花素组。灯盏花素组大鼠于术前1周分别ip不同剂量的灯盏花素(25,50 mg·kg^-1.d^-1),另2组给等量生理盐水。以左冠状动脉前降支穿线结扎法制备心肌缺血模型,松开结扎线造成再灌注。采用Annexinv-PI双染,流式细胞仪检测心肌细胞的凋亡情况;电镜观察各组大鼠心肌超微结构的变化。 结果 :灯盏花素可明显降低缺血再灌注大鼠心肌细胞的凋亡率(P<0.05,P<0.01),且较IR组心肌细胞超微结构损伤程度减轻。 结论 :灯盏花素对缺血再灌注心肌细胞具有保护作用,其机制可能是通过抑制心肌细胞凋亡实现的。

Abstract:

Objective: To explore the effect and mechanism of breviscapine against myocardial ischemia/reperfusion injury in rat. Method : 40 SD rats were randomly divided into sham operation group, ischemia/reperfusion group and breviscapine group. Rats in breviscapine group were injected with breviscapine 25, 50 mg·kg¹·d^-1 respectively prior to ischemia/reperfusion. The other two groups were given normal saline instead of breviscapine equally in volume at a week before the operation.The myocardial ischemia/reperfusion model was established by ligating the left anterior descending branch of coronary artery. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with AnnexinⅤand propidium iodiode (PI) staining. The ultrastructural alterations were observed under the transmission electron microscopy. Result: breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia/reperfusion injury obviously(P<0.05,P<0.01). The degree of injury on ultrastructures of myocardial cell in breviscapine group is rather alleviated compared with that in the ischemia/reperfusion group. Conclusion: breviscapine may protect myocardial cells during ischemia/reperfusion injury and the protective mechanism may be achieved by inhibiting myocardial apoptosis.