六味地黄汤、补中益气汤、复方丹参饮对骨髓抑制小鼠保护的作用机制

Effects of Liuwei Dihuang Decoction,Buzhong Yiqi Decoction and Compound Danshen Decoction on the Marrow-suppressed Mice

摘要:

目的 :进一步研究补肾阴、补气、活血中药方对骨髓抑制小鼠保护的作用机制。 方法 :使用实时荧光定量RT-PCR检测小鼠脾脏促血小板生成素(thrombopoietin,TPO) mRNA、促血小板生成素受体(c-Mpl)mRNA及转录因子GATA-1 mRNA表达水平。 结果 :六味地黄汤、补中益气汤均能明显升高骨髓抑制小鼠TPO,c-Mpl及GATA-1 mRNA的表达。复方丹参饮对小鼠脾脏TPO,c-Mpl及GATA-1 mRNA表达影响不明显。 结论 :六味地黄汤、补中益气汤均能通过升高骨髓抑制小鼠TPO,c-Mpl及GATA-1 mRNA的表达水平从而促进骨髓抑制小鼠的外周血恢复。

Abstract:

Aim: To study the protective therapeutic actions and mechanism of Liuwei Dihuang Decoction,Buzhong Yiqi Decoction and Compound Danshen Decoction on the marrow-suppressed mice. Method :The expression of thrombopoietin(TPO)mRNA, thrombopoietin receptor(c-Mpl)mRNA and GATA-1 mRNA was detected by RT-PCR technique. Result: Liuwei Dihuang Decoction and Buzhong Yiqi Decoction were shown to promote the expression of TPO mRNA, c-Mpl mRNA and GATA-1 mRNA in spleen of mice. The result indicates the decoctions could accelerate the marrow cells of the marrow-suppressed mice. Although Compound Danshen Decoction did not show significant promotion for the expression of TPO mRNA, c-Mpl mRNA and GATA-1 mRNA in spleen of mice, but it also could accelerate peripheral blood cells of the marrow-suppressed mice. Conclusion: Peripheral blood cells of marrow-suppressed mice were increased significantly by Liuwei Dihuang Decoction,Buzhong Yiqi Decoction and Compound Danshen Decoction.