忧虑康液对抑郁肾阴虚模型大鼠的滋肾阴作用机制研究

The Effect of You Lv Kang on the Model Rat of Kidney-Yin Deficiency and Depression Syndrome

摘要:

目的 :观察忧虑康液对肾阴虚抑郁模型大鼠行为学,血浆环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)以及足跖汗腺细胞形态学的影响。 方法 : 采用慢性不可预见性应激并ig甲状腺素加孤养法复制肾阴虚抑郁大鼠模型。将筛选的大鼠随机分为7组,造模同时ig给药,连续21 d,氟西汀组给予氟西汀3.6 mg ·kg^-1,地黄丸组给予六味地黄1.6 g(生药) ·kg^-1,忧虑康低、中、高剂量组分别给予忧虑康液7.2 g, 14.4,28.8 g(生药) ·kg^-1,正常组与模型组均ig等容量蒸馏水,以敞箱试验、液体消耗试验观察行为学,放免法检测血浆 cAMP、cGMP含量,HE染色法光镜下观察足跖汗腺细胞形态学变化。 结果 :模型大鼠饮水渐多而体重增长缓慢,敞箱试验和液体消耗试验有关指标显著下降,血浆 cAMP含量显著升高,足跖汗腺细胞发生明显空泡样变化、汗腺导管扩张。忧虑康液能改善模型大鼠阴液受伤及行为学异常,显著降低cAMP含量,并使足跖细胞形态及分泌的病理状况基本恢复正常;尤以高剂量与六味地黄丸作用相近。 结论 : 忧虑康液能调整β-受体系统,且呈量效关系,并能改善足跖汗腺细胞病理形态学变化;这些均是其滋阴作用机制之一。

Abstract:

Objective: To observe the effects of You Lv kang on depression behavior, plasma cyclic adenosine monophosphate(cAMP), cyclic guanosine monophosphate(cGMP), and cell morphology changes of plantar sweat gland cells in the model rat of kidey-Yin deficiency and depression syndrome. Method: Rats were divided into seven groups randomly. The rat model of kidney-Yin deficiency was established by exposing the animals to chronic unpredicable stress and giving thyroxine by intragastric adminstration. The model establishment and the treatment by different drugs were started at the same time. The dose for fluoxetine group was 3.6 mg ·kg^-1, the dose of Liuwei Dihuang Wan group was 1.6 g ·kg^-1. The three doses of You Lv Kang were 7.2 g ·kg^-1, 14.4 g ·kg^-1 and 28.8 g ·kg^-1 respectively. The blank and model groups were adminstrated with water daily. All the treatments lasted for 21 days.The behavior changs was evaluated by the open-field test and fluid comsumption test. The plasma samples of the rats were taken for cAMP and cGMP determination by radioimmunoassay. The morphology changes of plantar sweat gland cells with HE staining in rats were investigated under light microscope. Result: All the indexes of the open-field test and fluid comsumption test in the model rats were significantly decreased, with significantly increased the content of plasma cAMP. Vacuole-like changes in plantar sweat gland cells in the model rats were obvious, and sweat gland ducts were expanded. You Lv Kang could improve the behavior of the rats. The level of cAMP was significantly decreased in the Liuwei Dihuang Wan and You Lv Kang groups, and the morphological appearance of plantar sweat gland cells showed to be basically normal, especially in the high dose group of You Lv Kang and Liuwei Dihuang Wan group. Conclusion: You Lv Kang can regulate β-receptor system and improve the pathological changes of plantar sweat gland cell. Nourishing Yin might be one of the mechanisms responsible for the effects.