神昌注射液对脑栓塞大鼠血小板活化和血栓形成的影响

Effects of Shenchang Injection on Platelet Activation and Thrombosis in MCAO Rats

摘要:

目的: 观察神昌注射液对脑栓塞大鼠血小板活化和血栓形成的影响。 方法: 造大鼠大脑中动脉脑栓塞(MCAO)模型,静脉给药7次后,取血,用流式细胞仪测CD62P、CD63;并观察肾静脉血栓形成及凝血时间。 结果: 神昌注射液能降低脑缺血大鼠血液的CD62P、CD63表达率,降低大鼠下腔肾静脉血栓的重量,延长小鼠的凝血时间。 结论: 神昌注射液能降低血小板活化功能,减轻静脉血栓的形成,延缓血液凝固,是其治疗中风的药理学基础。

Abstract:

Objective: To observe effects of Shenchang Injection on platelet activation and thrombosis in MCAO rats. Method: The cerebral injury model induced by ischemia/reperfusion was made in rat by MCAO. Shenchang injection was given by iv for 7 times, then CD62p、CD63 were measured by flow-cytometry. Thrombosis and clotting time were observed. Result: Shenchang injection could decrease expression of CD62p and CD63 in blood, decline thrombosis in kidney vein, delay clotting time in MCAO rats. Conclusion: Shenchang injection is effect for antiplatelet activities and postponement of thrombosis by extending blood clotting. These are likely the pharmacological basis of the injection for treating stroke.