表没食子儿茶素没食子酸酯对脊髓损伤大鼠炎症因子释放及神经营养因子表达的影响

Effect of Epigaloctechin-3-gallit (EGCG) on the Release of Inflammatory Cytokines and Expression of Neurotrophic Factors in Spinal Cord-injuried Rats

摘要:

目的 :研究表没食子儿茶素没食子酸酯(epigaloctechin-3-gallit,EGCG)对脊髓损伤(spinal cord injury, SCI)大鼠炎症因子的释放及神经营养因子表达的影响。 方法 :建立大鼠脊髓半切SCI模型。SCI大鼠给予EGCG治疗24 h后取血清和受损伤脊髓局部组织。ELISA法测定血清白介素-6(IL-6)、白介素-8(IL-8)含量;Western blot法测定脊髓组织neurotrophin-3(NT-3)、brain-derived neurotrophic factor(BDNF)蛋白表达。 结果 :与模型组比较,EGCG能显著降低SCI大鼠血清炎性因子IL-6、IL-8的浓度,促进脊髓组织内源性NT-3、BDNF表达。 结论 :EGCG可抑制炎症因子释放,增加内源性神经营养因子表达。

Abstract:

Objective: To investigate the the protective effects of Epigallocatechin-3-gallate(EGCG) on the release of inflammatory cytokines and expression of neurotrophic factors in spinal cord-injuried(SCI) rats. Method: SCI model was reproduced by hemisection, and EGCG was administered for 24 h after hemisection 5 min after SCI. The concentrations of serum IL-6 and IL-8 were measured by ELISA assay, and the expression of neurotrophin-3(NT-3)and brain-derived neurotrophic factor(BDNF)protein in injured spinal cord tissues were determined by western blot. Result : EGCG significantly reduced the levels of serum IL-6 and IL-8, and increased expression of NT-3 and BDNF at the injured spinal cord in SCI rats. Conclusion : EGCG may protect against spinal cord from injury by inhibiting release of inflammatory cytokines and enhancing the expression of endogenous neurotrophic factors in SCI rats.