以X-5型大孔树脂吸附分离苦参生物碱的实验研究

Study on Adsorption and Separation of Alkaloids in Sophora flavescens ait. with X-5 Macroporous Resin

摘要:

目的: 研究一种环保、高效的苦参生物碱分离技术。 方法: 以苦参总碱吸附量和解吸率为指标,从大范围筛选树脂,并以选出的树脂进一步优化其吸附与解吸的条件。 结果: 所选出的树脂为X-5型非极性大孔树脂;优化的吸附条件为上柱液pH 10、盐离子浓度1 mol·L^-1,吸附流速6 BV·h^-1,总生物碱的吸附量可达9.74 mg·mL^-1;优化的洗脱条件为60%乙醇(pH 1)洗脱,解吸率可达95.5%。 结论: X-5型树脂能直接从苦参渗漉液中快速吸附分离生物碱,吸附量大、解吸容易,并对环境、产品无污染,极具开发成绿色工艺潜力。

Abstract:

Objective: To study a environmental and effective separation of alkaloids in sophora flavescens ait. Technology. Method: With the adsorption and desorption of total alkaloids in S. flavescens ait. as parameters,screening a resin from the large-scale,and optimizing condition of absorption and desorption of alkaloids in S. flavescens ait. Result: The nonpolarity macroporous resin X-5 were selected out;The optimized condition of adsorption is that sample solution pH 10, saltion concentration is 1 mol·L^-1, adsorption flow rate is 6 BV·h^-1. The adsorption is 9.74 mg·mL^-1; The optimized condition of desorption is that the alkaloids are desorpted by 60% ethanol (pH 1),the desorption is 95.5%. Conclusions: Macroporous resin X-5 can directly and fast adsorpte alkaloids from S. flavescens ait. percolation fluid, and desorption of alkaloids is easy, does not pollute products and environment,it has great potential for development of green technology.