高效液相色谱法测定广西余甘子叶中槲皮素和山奈酚的含量

RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L

摘要:

目的 :建立广西余甘子叶中槲皮素及山奈酚的含量测定方法。 方法 :采用HPLC法,色谱柱为Thermo Hypersil BDS C₁₈ (4.6 mm×250 mm,5 μm);流动相为乙腈-0.1%磷酸(27 ∶73);流速为1.0 mL·min^-1;柱温为30 ℃;检测波长为360 nm。 结果 :槲皮素在0.06~1.2 μg之间;山奈酚在0.021~0.43 μg,峰面积与进样量呈良好的线性关系;槲皮素和山奈酚的加样回收率分别为98.83%,101.58%,RSD分别为2.73%,2.21%(n=6)。 结论 :该方法简便可行,重复性好,结果准确,可作为广西余甘子叶中槲皮素和山奈酚的含量测定方法。

Abstract:

Objective: To establish a quantification method for quercetin and kaempferol in the leaves of Phyllanthus emblica by HPLC. Method: HPLC is applied on the determination of quercetin and kaempferol in the leaves of P. emblica with C₁₈ column(4.6 mm×250 mm,5 μm) and a mixture of acetonitrile-0.1% phosphoric acid (27:73) as a mobile phase with the flow rate at 1.0mL·min^-1. Quercetin and kaempferol were detected at 360nm, column temperature was set at 30 ℃. Result: Quercetin and kaempferol were linear in the range of 0.06~1.2 μg and 0.021~0.43 μg. The average recovery of quercetin and kaempferol was 98.83% with RSD 2.73% and 101.58% with RSD 2.21% (n=6) respectively. Conclusion: The method can be applied to the quality control of the leaves of P. emblica L.