高效液相色谱法测定止咳宁嗽胶囊中的绿原酸和橙皮苷

Simultaneous Determination of Chlorogenic acid and Hesperidin in Zhike Ningsou Capsule by HPLC

摘要:

目的 :建立高效液相色谱法同时测定止咳宁嗽胶囊中的有效成分绿原酸和橙皮苷的方法。 方法 :样品用甲醇超声提取,在Zorbax SB-C₁₈ (3.0 mm ×250 mm,5 μm)色谱柱上分离,以含1%乙酸的水(A)-乙腈(B)体系为流动相进行阶梯洗脱,程序为0 min(82 ∶18)~5 min(82 ∶18)~5.1 min(75 ∶25)~20 min(75 ∶25);流速为0.35 mL ·min^-1;327 nm紫外检测。 结果 :绿原酸在进样量为0.4~10.0 μg ·mL^-1内呈良好的线性关系;橙皮苷在进样量为6.2~155.0 μg ·mL^-1内呈良好的线性关系。绿原酸的平均回收率为 99. 2%,RSD为 1.0%。橙皮苷的平均回收率为 99. 6%, RSD为 0.9%。 结论 :该方法简便快速,结果准确,重复性好,可为止咳宁嗽胶囊的质量控制提供参考依据。

Abstract:

Objective :To establish a HPLC method for simultaneous determination of chlorogenic acid and hesperidin in Zhike Ningsou Capsule . Method :The sample was extracted with methanol under ultrasonication. The HPLC separation was performed on a Zorbax SB C₁₈ (3.0 mm ×250 mm,5 μm) column,using water containing 1% acetic acid(A) –acetonitrile(B) as the mobile phase,in stepwise elution:0 min(82 ∶18)~5 min(82 ∶18)~5.1 min(75 ∶25)~20 min(75 ∶25),at a flow rate of 0.35 mL ·min^-1. Result :The linear range was 0.4~10.0 μg ·mL^-1 and 6.2~155.0 μg ·mL^-1 for chlorogenic acid and hesperidin; the average recovery was 99.2% and 99.6%; RSD 1.0% and 0.9%,respectively. Conclusion :This method can be applied for the quality control of Zhike Ningsou Capsule.