5种贝母的弱极性成分HPLC指纹图谱研究

Study the Low-polarity Parts of Five Fritillaria Species by HPLC Fingerprints

摘要:

目的 :建立5种贝母弱极性成分的HPLC指纹图谱,以此鉴别贝母类药材,并对不同种的贝母加以区分。 方法 :以三相静态萃取法提取制备样品,采用高效液相色谱法,以乙腈-0.1%磷酸为流动相进行梯度洗脱;检测波长250 nm;记录时间60 min;以中药色谱指纹图谱相似度评价系统软件分析图谱。 结果 :建立了5种贝母弱极性成分的HPLC指纹图谱,标定了16个共有指纹峰及各自的特有峰,方法学考察符合指纹图谱技术要求。相似度计算表明,5种贝母弱极性成分的指纹图谱存在一定的差异。 结论 :该实验建立的HPLC图分离度好、信息量大、基线平稳,为5种贝母的鉴别和质量控制提供了可靠的分析方法。

Abstract:

Objective: To establish the HPLC fingerprint of five Fritillaria species according to the low-polarity for the identification. Method: Samples was prepared with static three-phase extraction, HPLC was used the gradient eluted solvert system composed of CH₃CN-0.1%H₃PO₄, the flow rate was 1.0 mL·min^-1, the detection wavelength was 250 nm. The HPLC fingerprints were evaluated by similarity evaluation system. Result: Published the fingerprints of five Fritillaria species, determined 16 common peaks and special peaks of five Fritillaria species. The result of similarity indicated that differences exist in five Fritillaries species. Conclusion: The established HPLC fingerprint has desirable accuracy, repeatability and stability, it can be used for one of the quality control of Fritillaria species.