全蝎蛋白药效组分的生物鉴定法研究

Study the Bioassay Method of Informational Active Components Alignment of Scorpion

摘要:

目的 :研究全蝎蛋白药效组分的提取方法,摸索全蝎蛋白药效组分SDS-PAGE的最佳电泳条件,测定全蝎蛋白药效组分电泳图谱条带的大致分子质量,确定其SDS-PAGE的表达特征,从而建立全蝎蛋白药效组分的电泳鉴别法。 方法 :采用水提与盐析法获得全蝎蛋白药效组分,考马斯亮蓝法测定蛋白药效组分的蛋白质含量,SDS-PAGE法和Alpha Ease FC 4.0.0软件测定全蝎蛋白药效组分的蛋白质相对分子质量。 结果 :全蝎蛋白药效组分的蛋白质质量分数为50.37%;得到较好的全蝎蛋白药效组分的10条电泳谱带,相对分子质量分别为94.162 0,66.400 0,40.357 0,27.541 0,22.518 0,19.520 0,12.766 0,11.190 0,9.665 3,8.534 2 KDa。 结论 :这10条谱带是全蝎蛋白药效组分的特征谱带,可用于全蝎蛋白药效组分的鉴别。

Abstract:

Objective: We studied the extracted method of informational active components alignment of scorpion, groped the best electrophoresis conditions, determined the molecular mass of informational active components alignment, assured the expressing characteristic of the electrophoretic bands, and set up the bioassay method of informational active components alignment of Scorpion. Method: We extracted scorpion by water and salt fractionation to obtain informational active components alignment, Coomassie brilliant blue method determined its protein content, and SDS-PAGE electrophoretic technique and Alpha Ease FC 4.0.0 software determined its protein molecular weight. Result: The protein content of informational active components alignment was 50.37%. There were ten bands in the informational active components alignment. Ten bands in the SDS-PAGE were 94.162 0,66.400 0,40.357 0,27.541 0,22.518 0,19.520 0,12.766 0,11.190 0,9.665 3,8.534 2 KD. Conclusion: These ten bands were the characteristic bands of informational active components alignment, and might be used to identify the informational active components alignment.