增液汤降糖作用实验研究

Experimental Study on Hypoglycemic Effects of Zengyetang

摘要:

目的 :研究增液汤的降糖作用及其作用途径。 方法 :采用正常小鼠和四氧嘧啶(70 mg ·kg^-1)诱导的糖尿病模型小鼠,连续给药7 d后,观察增液汤水提物(5, 10,15 mg ·kg^-1)对血糖、口服糖耐量、血清胰岛素水平和体重的影响;采用MIN6胰岛细胞观察增液汤水提物(12.5, 25, 50,100 μg ·mL^-1)对胰岛素分泌的影响。 结果 :增液汤可明显降低糖尿病小鼠的血糖,改善正常小鼠和糖尿病小鼠的糖耐量,促进胰岛素分泌;增液汤水提物在高糖(16.7 mmol ·L^-1)环境下能够明显促进MIN6胰岛细胞分泌胰岛素。 结论 :增液汤能降低糖尿病小鼠的血糖,改善正常小鼠和糖尿病小鼠的糖耐量,其作用机制可能与促进胰岛素的分泌有关,与磺酰脲类降糖药的作用途径不同。

Abstract:

Objective: To investigate the effects and related pathways of Zengyetang on lowering blood glucose. Method: The normal mice and alloxan (70 mg ·kg^-1)-induced diabetic mice were administrated for 7 d in order to investigate the effects of aquatic extract of Zengyetang (5, 10 and 15 mg ·kg^-1) on the levels of blood glucose, insulin and oral glucose tolerance. Then we investigated the effects of extract (12.5, 25, 50,100 μg ·mL^-1) on insulin secretion in MIN6 islet cells. Result: After 7 days of administration, the extract could significantly lower blood glucose, and it also could ameliorate the oral glucose tolerance in normal and diabetic mice and improve insulin secretion. Meanwhile, the extract could improve insulin secretion in MIN6 cells with stimulation of 16.7 mmol ·L^-1 glucose. Conclusions: The Zengyetang could lower blood glucose and ameliorate the oral glucose tolerance through improving insulin secretion, whose pathways were maybe different from sulphonylurea hypoglycemic drugs.