大分子葡萄糖酐血瘀大鼠偶氮蓝体表特征 与血液流变性的相关性

Relativity Between the Body Surface Characteristics Indicated by Evans Blue and the Hemorrheological Parameters in Rats with Blood-stasis Syndrome Induced by Macromolecule Dextran

摘要:

目的 :探讨大分子葡萄糖酐血瘀证大鼠表征的偶氮蓝变化特征与血液流变性的相关性。 方法 :分别对大鼠尾iv 0.5%偶氮蓝配制的10%大分子葡萄糖酐溶液,0.5%偶氮蓝溶液,生理盐水配制10%的大分子葡萄糖酐溶液,或生理盐水,在注射后30,60,120,240 min观察和拍摄大鼠耳廓、舌体表征变化,检测血液流变性,分析体表特征变化与血液流变学指标间的相关性。 结果 :①尾iv 0.5%偶氮蓝配制的10%大分子葡萄糖酐溶液的大鼠30 min时耳廓和舌出现蓝色斑点,并随时间延长,蓝色瘀斑范围增大,240 min时耳廓完全呈深蓝色,舌尖蓝色瘀斑范围继续增加,几乎占舌体1/2;而只注射0.5%偶氮蓝的大鼠对照组耳廓始终半透明,舌体颜色均匀。②iv生理盐水配制10%的大分子葡萄糖酐溶液的大鼠与只注射生理盐水的大鼠相比,30,60 min不同减切速率下全血黏度均显著增高,30,60,120,240 min血浆黏度、红细胞聚集指数、聚集指数积分也均显著增高,而30,60,120,240 min的红细胞变形面积指数、变形指数积分面积虽未测出明显差异,但趋势表现为降低。 结论 :大鼠尾iv 0.5%偶氮蓝配制的10%大分子葡萄糖酐后能使血瘀证模型大鼠的血瘀程度在其耳廓和舌体表征中较为直观地呈现,且与注射大分子葡萄糖酐后血液流变性变化所反映的血瘀程度相一致,并呈明显的时效关系,进一步说明偶氮蓝可以客观反映大分子葡萄糖酐所致血瘀证模型大鼠血瘀程度的表征变化。

Abstract:

Objective: To investigate the relativity between the body surface characteristics indicated by Evans blue and the hemorrheological parameters in rats with blood-stasis syndrome induced by macromolecule dextran. Method: The rats were treated by injected 10% macromolecule dextran solution confected by 0.5% Evans blue, 10% macromolecule dextran solution confected by sodium chloride, 0.5% evans blue, or sodium chloride respectively through the caudal vein, then observed and photoed the exosymptom change presented by Evans blue in ear and tongue of the rats, and detected the hemorrheologic variation of the rats at 30, 60, 120, and 240 minutes after the treatment, and analyzed the relativity between the body surface characteristics indicated by Evans blue and the hemorrheological parameters in rats with blood-stasis syndrome. Result: In the rats treated by 10% macromolecule dextran solution confected by 0.5% Evans blue, the blue petechia in rat auricle and tongue were observed after 30 minutes, which aggravated as the time went by, and the whole ear of rat changed to dark blue and the blue petechiae of tongue enlarged to more than half of tongue after 240 minutes, while in the rats treated by 0.5% Evans blue,the rat auricle was semitransparent and the tongue color was uniform. In therats treated by 10% macromolecule dextran solution confected by Sodium Chloride, the blood viscosity increased significantly in different shear rate in 30 and 60 minutes, and the plasma viscosity, the aggregation index of erythrocyte, and the integral of aggregation index significantly increased at 30, 60, 120, and 240 minutes compared with the rats injected by sodium chloride. The index of red blood cell deformation and the integral area of deformation index at 30, 60, 120, and 240 minutes in the rats injected by sodium chloride are slightly lower than that in rats treated by 10% macromolecule dextran solution confected by sodium chloride, though there was no significantly difference can be detected. Conclusion: The surface characteristics in rat model with blood-stasis syndromcan be observed directly through the exosymptom change of ear and tongue when 0.5% Evans blue was confected into the 10% macromolecule dextran solution, and it correlated with the degree of blood stasis showed by the hemorrheologic variation. It indicates that the exosymptom changes in rats with blood-stasis syndrome induced by macromolecule dextran can be reflected objectively by Evans blue.