微透析法进行盐酸青藤碱肌注给药的药代动力学研究

Pharmacokinetics Study on Sinomenine Hydrochloride in Rats with Administration route of Intramscular Injection by Using Microdialysis Method

摘要:

目的 :微透析法进行盐酸青藤碱肌注给药的药代动力学研究。 方法 :微透析探针植入大鼠的右心室,实时、活体采集肌注盐酸青藤碱后的透析液样品,HPLC检测样品浓度,微透析样品浓度经校正后进行房室和非房室模型拟合。 结果 :盐酸青藤碱肌注后C_max为3.127 mg·L^-1,T_max为101 min,MRT为191 min,结果表盐酸青藤碱肌注后血药浓度呈一级速率、一室模型。 结论 :微透析法能较好的反映盐酸青藤碱肌注给药的药动学特征,微透析采样手段可作为药动学研究的理想手段。

Abstract:

Objective: To explore the pharmacokinetics of sinomenine hydrochloride by using microdialysis method. Method: The microdialysis probe was introduced to right ventricle of rats as sampling tools for real-time and in vivo collecting samples after intramscular injection. The dialysate samples were determined by HPLC. After the dialysate concentration was calibrated, the blood-drug concentration was fitted with compartment and noncompartment model. Result: The results shows that physiological disposition of Sinomenine Hydrochloride was fitted to first order rate and open one-compartment model. The main pharmcokinetics parameters were as following: C_max 3.127 mg·L^-1,T_max 101 min,MRT 191 min. Conclusion: The pharmacokinetics characteristics of Sinomenine Hydrochloride was collected by using microdialysis method which could been selected as study tool.