莪术油对肺腺癌A549细胞周期及组织蛋白酶K表达的影响

Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line

摘要:

目的 :探讨莪术油对A549细胞增殖抑制作用及组织蛋白酶K表达的影响。 方法 : MTT法观察莪术油对A549细胞增殖的抑制作用;流式细胞术检测细胞周期阻滞;Western blot检测组织蛋白酶K的表达情况。 结果: 莪术油作用后①MTT法结果显示莪术油在60 mg ·L^-1~200 mg ·L^-1对A549细胞增殖有抑制作用, IC50随作用时间的延长显著前移。②流式细胞术结果出现凋亡峰,与未处理组细胞相比,给药组细胞G0/G1期细胞比例增加,S期细胞比例降低。③Western blot结果显示,莪术油处理细胞24 h后,组织蛋白酶K表达明显上调,100 mg ·L^-1达最大值。 结论 : 莪术油能抑制肺腺癌A549细胞增殖,阻滞细胞周期的作用,其机制可能与上调组织蛋白酶K表达水平有关。

Abstract:

Objective: To explore the Zedoary oil on A549 cell line inhibiting the proliferation and cathepsin K expression. Method : The effects of Zedoary oil on the proliferation of lung adenocarcinoma cell line A549 were determined by MTT assay. The cells cyclic retardation were examined by using flowcytometry. Cathepsin K expression of level were detected by using western blot. Result : MTT showed that Zedoary oil had an inhibitory effect on the proliferation of A549 cells in 60~200 mg ·L^-1. Flow cytometry showed that Zedoary oil increased the percentage of G₀/G₁ phase cells and reduced the percentage of S phase cells. The results of western blot showed that the expression of cathepsin K was up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls. Conclusion :Zedoary oil can significantly inhibit the growth of A549 cells,blocking the cell cycle. The effects may due to the up-regulation of cathepsin K.