三七绞股蓝药对及其总皂苷配伍对兔动脉粥样 硬化模型主动脉的影响

Effects of Panax notogiuseng(PN) and Gynostemma pentaphyllum(Gp) on Aorta in Rabbit with Experimental Atherosclerosis

摘要:

目的 :观察三七绞股蓝药对及其总皂苷配伍对兔动脉粥样硬化模型血脂及主动脉的影响。 方法 :取40只雄性新西兰兔,随机分为5组:正常对照组、三七绞股蓝饮片组、三七绞股蓝皂苷组、阳性药物组、模型对照组。除正常组给予普通兔饲料,其余各组给予高胆固醇饲料并注射牛血清白蛋白,在造模的同时预防给药。12周后测定血脂,用计算机图象分析系统观察动脉损伤后血管重构相关指标的变化。 结果 :三七绞股蓝饮片可降低平滑肌细胞核密度百分数(VSMC)水平,减小中膜面积(P<0.01);三七绞股蓝皂苷组可减小中膜面积(P<0.05);三七绞股蓝饮片组、三七绞股蓝皂苷组均能增大管腔面积(P<0.01),减小内膜面积(P<0.01),减少内膜厚度(P<0.01)。 结论 :三七绞股蓝药对及其总皂苷配伍具有抗血管重构的作用。

Abstract:

Objective: To investigate the effects of Panax notogiuseng(PN) , Gynostemma pentaphyllum(Gp) and their total saponins(PNs and Gps) on vascular remodeling in experimental atherosclerosis rabbit and to explore the possible mechanism. Method: Forty healthy male Zelanian white rabbits were used in the experiment. They were randomly selected and allocated as 5 groups:group 1 nomlal control group, group 2 treated with PN and Gp, group 3 treated with PNs and Gps, group 4 treated with captopril , group 5 positive control group. Normal control group were fed with normal diet, the other groups were fed with hypercnolesterol diet. After administered for 12 weeks,histopathological examination of abdominal aorta with the most obvious lesion was performed, and the changes of related vascular remodeling indexes were observed using computerized image analyzing system. Result: PN and Gp could significantly decreased the number of VSMC(P<0.01). The area of medial membrane of group 2 were significantly lower than that in the positive control group. Conclusions: PN,Gp and their total saponins played its action in preventing and treating atherosclerosis through inhibiting intimal proliferation and intervening pathological vascular remodeling.