痛泻要方组成药物对大鼠结肠平滑肌运动的影响及机制

Effect and Mechanisms of Composition of Tongxie Yaofang on in vitro Contraction of Colonic Smooth Muscle Strips of Rat

摘要:

目的 :观察痛泻要方拆分成分防风(RS)、白术(LA)和白芍(WPR)对离体大鼠结肠平滑肌的影响,并探讨其部分机制。 方法 : 制备大鼠结肠平滑肌肌条,以9 g ·L^-1 NaCl溶液为对照,观察白术、白芍和防风对平滑肌自发收缩的影响和防风对乙酰胆碱(Ach)引起的肌条收缩的影响,并用3种工具药(纳络酮(NLT)、心得安(XDA)、酚妥拉明(FILM))初步探讨了防风的作用机制。 结果: 与NS组相比较,白术、白芍均对大鼠平滑肌有促进收缩的作用,且白术的作用大于白芍;防风各剂量组(10~40 g ·L^-1)均对平滑肌收缩有剂量依赖性的抑制作用。不同浓度的防风对Ach引起的肌条收缩作用均有抑制,并呈剂量依赖关系。先用工具药充分孵育后再加入RS,与对照组(NS+RS)比较,NLT+RS组面积的回升有统计学意义(P<0.05);FTLM组振幅和面积回升差异均有统计学意义(P<0.01),与阴性对照组(NS)的差异则无统计学意义。 结论: 痛泻要方主要成分白术和白芍对大鼠结肠平滑肌均有促进收缩的作用。防风有抑制作用,其作用机制与肾上腺素能α受体有关,与胆碱能M受体可能也有一定关系。

Abstract:

Objective: To determine the in vitro effect of Radix Saposhnikoviae (RS), Largehead Atractylodes Rh (LA) and white paeony root (WPR) on the colonic smooth muscle strips of rats and study the possible mechanisms. Method: The colonic longitudinal smooth muscle strips of rats were prepared. Sodium chloride (9 g ·L^-1) was used as the control groups. We observed the effects of WPR, LA and RS on the spontaneous contraction of the muscle strips. To study its mechanisms further, we also observed the effects of three tool drugs (naloxone, propranolol and phentolamine) on the strips. Result: Compared with the control group, both LA and WPR had the promoting role on the in vitro contraction of colonic smooth muscle strips. In addition, the activity of LA was stronger than WPR. RS of different concentrations showed dose-dependent inhibitive effects on 10~40 g ·L^-1 in the spontaneous contraction of colonic muscle strips. When acetylcholine was Added, RS showed dose-dependent inhibitive effects on the contraction of muscle strips. Compared with the NS+RS group, the area of NLT+RS group had significant difference (P<0.05), so were the peak and area of FTLM+RS group (P<0.01), while NS group was insignificant. Conclusion: RS showed inhibitive effect on the spontaneous in vitro contraction of colonic smooth muscle strips of rats, while LA and WPR showed promoting effect. The mechanisms of inhibition are supposed to have close relations with the α-adrenoceptor and maybe also related with the M-cholinergic receptor.