参附汤、芪附汤、姜附汤对阿霉素心脏毒性损伤 大鼠线粒体途径细胞凋亡的影响

Effects of Shenfu Decoction, Qifu Decoction, Jiangfu Decoction on Cardiomyocyte Apoptosis Through Mitochondrion Signaling Pathway of Cardial Injury Model Induced by Adriamycin in Rat

摘要:

目的 :探讨参附汤、芪附汤、姜附汤对阿霉素心脏毒性损伤大鼠线粒体途径细胞凋亡的影响。 方法 :采用酶联免疫测定法(ELISA)测定心肌线粒体抗凋亡基因(Bcl-2),促凋亡基因(Bax),细胞色素c(cytc),半胱氨酸天冬氨酸蛋白酶9(Caspase-9), 半胱氨酸天冬氨酸蛋白酶3(Caspase-3)含量。 结果 :阿霉素组心肌线粒体Bcl-2含量降低、Bax含量提高,cytc,Caspase-9,Caspase-3含量上升,均有统计学意义(P＜0.05)。参附汤、芪附汤、姜附汤3组心肌线粒体Bcl-2含量提升、Bax含量下降,cytc,Caspase-9, Caspase-3含量下降,均有统计学意义(P＜0.05)。 结论 :Bcl-2,Bax, cytc,Caspase-9,Caspase-3与阿霉素心脏毒性损伤发生、发展过程有关,参附汤、芪附汤、姜附汤通过抑制线粒体途径的细胞凋亡保护心肌抑制阿霉素心脏毒性损伤。

Abstract:

Objective: To investigate the effect of Shenfu Decoction, Qifu Decoction, Jiangfu Decoction on cardiomyocyte apoptosis through the mitochondrion signaling pathway of cardial injury model induced by adriamycin in rat. Methods : Enzyme-linked immunosorbent assay(ELISA) was applied to measure the content of Caspase-9, Caspase-3, Bcl-2, Bax and cytochrome C in cardiomyocyte mitochondria. Results : In adryamycin-induced rat group: the content of Bcl-2 in myocardial mitochondria decreased, the content of Bax increased, the content of Caspase-9, Caspase-3 and cytochrome C increased, with statistical significance (P<0.05). In Shenfu Decoction group, Qifu Decoction group and Jiangfu Decoction group: the content of Bcl-2 in myocardial mitochondria increased, the content of Bax decreased, the content of Caspase-9, Caspase-3 and cytochrome C decreased, with statistical significance (P<0.05). Conclusion : There is relationship between the content of Caspase-9, Caspase-3, Bcl-2, Bax and cytochrome C and the ocurrence and development of adriamycin-induced cardial injury. Shenfu Decoction, Qifu Decoction, Jiangfu Decoction can protect myocardium by inhibiting adriamycin-induced cardiomyocyte apoptosis through the mitochondria signaling pathway.