利心冲剂对心衰大鼠模型的治疗作用和毒理学研究

Therapeutical Effect and Toxicologic Study of Lixin Granule on Adriamycin-induced Heart Failure Rats ZHUANG Mei\|fang1, GAO Xiang2*, ZHANG Feng\|li2, SHI Mei\|qing1, JIANG Feng\|rong3

摘要:

目的 :研究利心冲剂对心衰大鼠治疗作用及药物毒理学。 方法 :实验分组:正常对照组、模型组、利心冲剂低剂量组、利心冲剂高剂量组、卡托普利阳性药组。腹腔注射阿霉素(ADR)5 mg·kg^-1,每5 d 1次,共3次,复制大鼠心衰模型,左心室插管术测定血流动力学指标,HE病理染色;毒理学研究测定大鼠LD₅₀和最大耐受量,另予利心冲剂4,8,16 g·(100 g)^-13个剂量组和空白对照组,连续8周口服给药,测定大鼠体重,于停药时及停药后7 d进行血液常规、肝功能、肾功能检查,实验结束处死动物,计算脏器系数,进行病理学检验。 结果 :利心冲剂治疗组和模型组比较,左心室收缩内压显著升高,形态学显示心肌细胞变性坏死明显减轻;毒性试验表明,以成人口服剂量的60倍经口灌胃,未见大鼠明显毒性反应;长期应用对大鼠体重、血常规、肝功能、肾功能和脏器系数、病理均无影响。 结论 :利心冲剂能改善心脏功能,具有较高的安全性。

Abstract:

Objective: To observe the The therapeutical effect and toxicologic study of Lixin granule on the adriamycin-induced heart failure rats. Method: sixty adult male Wistar rats were divided into 5 groups: normal control group, adriamycin(ADR) group,L- Lixin granule group, H- Lixin granule group, captopril group. Then ADR of 5 mg·kg^-1 was given intraperitoneally once to copy the model of heart failure once 5 days, totally 3 frequency. Objective to monitor the hemodynamic index on the adriamycin-induced heart failure rats through left ventricle intubatton.Pathological changes were observed by HE staining.The LD50 and maximum tolerance of heart failure rats were determined on toxicologic study.Another three-dose group )^-1] and blank control group were oral administrated.8 weeks later, the body weight were measured.The conditions of blood, the liver and renal functions were determined after treatment as well as following seven days. The rats were sacrificed after the experiment.Meanwhile,the coefficient of organ was calculated and the pathology was also tested. Result: As compared with ADR group,the LVSP the Lixin granule group were significantly higher and HE staining showed that the myocardial tissue damage were significantly improved. By tests of mouse toxicity, sixtyfold-weight Lixin granule than sdult is revealed that the rats was orally administered through a stomach and Lixin granule is an actually nonpoisonous material. After a long period of time,the weight, routine blood test, liver , kidney function and main viscera of rats were not influenced obviously. Conclusion: Lixin granule treatment can improve the cardiac contractility of adriamycin-induced heart failure rats; and also obviously be provided with better security.