金边瑞香正丁醇提取物抗肿瘤作用及机制的研究

Anti-tumor Effect of N-Butanol Extract of Daphne odora var. Marginata and its Mechanism

摘要:

目的 :探讨金边瑞香正丁醇提取物的抗肿瘤作用及其机制。 方法 :采用萃取法对金边瑞香浸膏进行提取,利用小鼠皮下移植性肿瘤模型观察不同浓度金边瑞香正丁醇提取物的体内抗肿瘤作用; 采用MTT比色法测定NK细胞活性;ELISA法测定小鼠脾细胞分泌IL-2的量。 结果 :1.金边瑞香正丁醇提取物对荷S180小鼠的肿瘤生长有抑制作用,10,20,40 g ·kg^-1 ·d^-1剂量组抑瘤率分别达到34.90%,47.16%,29.72%。2.金边瑞香正丁醇提取物各剂量组的小鼠脾细胞分泌的IL-2均有提高,其中20 g ·Kg^-1 ·d^-1剂量组IL-2为(5.26±0.61)pg ·mL^-1显著高于生理盐水组并明显增强NK细胞活性。 结论 :金边瑞香正丁醇提取物在体内具有显著的抗肿瘤活性,且可促进脾细胞分泌IL-2和增强NK细胞活性。

Abstract:

Objective: To investigate anti-tumor effect and possible mechanism of N-butanol extract of Daphne odora Var.Marginata in vivo. Method: Solvent extraction method was used for obtaining extraction from D. odora. Mouse model of transplanting tumor was used to study the in vivo anti-tumor effect of N-butanol extract of D. odora at different concentrations. MTT method was used to examine the effect of NK cell. ELISA method was used to examine IL-2 secreted by spleen cell. Result : N-butanol extract of D. odora could decrease the tumor weight in S180-bearing mice. It could raise IL-2 secreted by spleen cell, and obviously enhance the effect of NK cell. Conclusion: N-butanol extract of D. odora has anti-tumor activity in vivo and promote spleen cell secrete IL-2 and enhance the effect of NK cell.