头花蓼对α-葡萄糖苷酶的抑制活性研究

Inhibitory Activity of Polygonum Capitatum to α-Glucosidase

摘要:

目的 :研究头花蓼不同溶剂提取物对 α -葡萄糖苷酶的抑制活性。 方法 :采用索氏提取法对头花蓼进行不同溶剂提取,以体外法测定各提取物对 α- 葡萄糖苷酶的抑制作用。 结果: 头花蓼各提取物均具有较好的 α -葡萄糖苷酶抑制活性。其中头花蓼的甲醇提取物抑制活性最高(IC₅₀ 1.68 μg·mL^-1),乙酸乙酯提取物(IC₅₀ 7.27 μg·mL^-1)和石油醚提取物(IC₅₀ 116.81 μg·mL^-1)的活性次之。3个提取物活性均远大于阳性对照acarbose(IC₅₀ 1 081.27 μg·mL^-1)。且各提取物对 α- 葡萄糖苷酶的抑制作用均具有剂量依赖性。 结论: 头花蓼甲醇提取物对 α- 葡萄糖苷酶活性的抑制效果非常显著,具有很好的开发价值。

Abstract:

Objective: To investigate different extracts of Polygonum capitatum on inhibitory activity to α -glucosidase. Method: Different extracts were extracted by Soxhlet, and the inhibitory activity to α- glucosidase was screened by the 96-microplate-based method. Results: The results showed that P. capitatum had good inhibitory activity. The methanol extract had the highest inhibitory activity to α -glucosidase (IC₅₀ 1.68 μg·mL^-1), and then the ethyl acetate extract (IC₅₀ 7.27 μg·mL^-1) and the petroleum ether extract (IC₅₀ 116.81 μg·mL^-1), three extracts of P. capitatum showed higher activity than Acarbose (IC₅₀ 1 081.27 μg·mL^-1). And all of the extracts showed dose dependent for the inhibitory activity. Conclusion: The methanol extract of P. capitatum showed very significant inhibitory activity to α -glucosidase, and could be exploited the diabetes drugs as α- glucosidases inhibitors in future.