交泰丸浸膏粉溶液对豚鼠心室肌细胞膜电位的影响

Effect of Jiaotaiwan on the Membrane Potentials of Ventricular Myocytes in Guinea Pig

摘要:

目的 :观察交泰丸(JTW)浸膏粉溶液对豚鼠心室肌细胞膜电位的影响。 方法 :使用Langendorff灌流系统离体灌流心脏,急性酶解法分离获得单个心室肌细胞。全细胞膜片钳技术电流钳模式记录膜电位。 结果 :0.2%交泰丸浸膏粉使静息电位(RP)从用药前(-85.91±6.24)mV下移至(-99.64±6.62)mV(n=8,P＜0.01),0相除极幅度(APA)从用药前(128.91±1.39)mV上升至(139.74±2.67)mV(n=8,P＜0.01),复极至50%时动作电位时程(APD₅₀)从用药前(505.49±30.97)ms缩短至(329.94±44.32)ms(n=8,P＜0.01),复极至90%时动作电位时程(APD₉₀)从用药前(524.96±30.99)ms缩短至(358.41±43.51)ms(n=8,P＜0.01),复极至50%到复极至90%的动作电位时程(APD_50-90)从用药前(16.56±1.48)ms延长至(27.31±3.62)ms(n=8,P＜0.01),动作电位3相复极速率(V₃)从用药前(-2.93±0.33)V/s减慢至(-1.95±0.28)V/s(n=8,P＜0.01)。0.1%,0.4%交泰丸浸膏粉对各项指标的影响与0.2%交泰丸浸膏粉的变化趋势一致。 结论 :交泰丸浸膏粉引起心室肌细胞RP下移,APA上升,APD₅₀缩短,APD₉₀缩短,APD_50-90延长和V₃减慢。以上影响可能是其在临床上具有抗心律失常作用的电生理学机制。

Abstract:

Objective: To investigate the effect of Jiaotaiwan on action potentials(AP) of isolated ventricular myocytes in guinea pig. Method: The myocytes were enzymatically obtained from the guinea pig hearts and the action potentials were examined by whole-cell patch clamp technique. Result: Jiaotaiwan (0.2% solusion) decreased the resting potential from (-85.91±6.24)mv to (-99.64±6.62) mv (n=8,P＜0.01), increased action potential amptitude(APA) from (128.91±1.39)mv to (139.74±2.67)mv(n=8,P＜0.01), shortened action potential duration of repolarization 50%(APD₅₀) from (505.49±30.97)ms to (329.94±44.32)ms (n=8,P＜0.01), shortened action potential duration of repolarization 90%(APD₉₀) from (524.96±30.99)ms to (358.41±43.51)ms (n=8,P＜0.01), extended action potential duration of repolarization 50% to repolarization 90%(APD_50-90) from (16.56±1.48)ms to (27.31±3.62)ms (n=8,P＜0.01), and slowed down the slope of phase 3(V₃) from (-2.93±0.33)v.s^-1 to (-1.95±0.28) v.s^- (n=8,P＜0.01). 0.1% and 0.4% Jiaotaiwan on the various indicators of impact and 0.2% of the change in trend. Conclusion: Jiaotaiwan can induce the following influence for ventricular myocytes: decreased RP, increased APA, shortened APD₅₀, shortened APD₉₀, extended APD_50-90 and V₃ slowed down. The influences of Jiaotaiwan are likely linked with the electrophysiological mechanism of its anti-arrhythmic effect.