清热解毒方对脓毒症大鼠静脉血细胞黏附分子的影响

Effects of Qingrejiedu Fang on Expression of Multiple Adhesion Molecules in Septic Rat Model

摘要:

目的 :探讨清热解毒方对脓毒症大鼠血液中淋巴细胞、单核细胞和中性粒细胞黏附分子表达的影响。 方法 :采用盲肠结扎穿孔法制备脓毒症大鼠模型。将Wistar大鼠分为正常对照组、模型组、清热解毒方组(分为高、低两个剂量组),采用流式细胞术分别观察各组血液中免疫细胞数量的变化和细胞表面黏附分子CD11b/c,CD62L和CD54表达的情况。 结果 :模型组大鼠血细胞CD11b/c,CD54表达增高,而CD62L表达降低,与正常组比较有统计学差异(P<0.05),与模型组比较清热解毒方组CD11b/c,CD54的表达显著降低(P<0.05),而CD62L的表达显著升高(P<0.05)。 结论 :清热解毒方能调整血液中多种细胞黏附分子的异常表达,改善机体的免疫状态。

Abstract:

Objective: To study the effects of Qingrejiedu Fang on expression of multiple adhesion molecules in septic rat model . Method: Using a sepsis model induced by cecal ligation and puncture (CLP) in rat , the septic rats were treated with 2 different doses of Qingrejiedu Fang for 72 hours. The expressions of CD11b/c, CD62L and CD54 of circulating blood cells were determined by flowcytometry. Result: The expressions of CD11b/c and CD54 were increased significantly compared with the control group, while the CD62L was decreased.The Qingrejiedu Fang could reverse this tendency. Conclusion: The high expressions of adhesion molecules and their adhesive effects play an important role in the pathological process of multiple organ dysfumctions in sepsis and the Qingrejiedu Fang has therapeutic effects on septic model by regulating the expressions of multiple adhesion molecules.