红参中人参总皂苷的大孔树脂纯化工艺

Purification of Total Saponins from Panax Ginseng by HPD Macroporous Resins

摘要:

目的: 优选最佳的分离纯化红参中人参总皂苷的工艺条件。 方法: 以人参总皂苷的量为指标,比较不同型号树脂和不同工艺条件对人参总皂苷的分离纯化能力。 结果: 确定HPD-400大孔树脂为人参总皂苷的纯化材料,其最佳工艺条件是药液质量浓度1.7 g ·L^-1,药液pH 4.68,吸附速度7.5 BV ·h^-1,用4 BV80%乙醇以7.5 BV ·h^-1的速度洗脱人参总皂苷。 结论: 本工艺条件可以用于含有人参总皂苷的中药制剂的制备提供依据。

Abstract:

Objective: To study the optimum methods of extracting and refining ginsenosides from red ginseng. Method: The ability of extracting and refining ginsenosides was compared in different types of macroporous resin and different technological conditions with total amount of ginsenosides as index. Result: The HPD-400 macroporous resin was best for separating and purifying total saponins in the following technological conditions: the sample concentration was 1.7 g ·L^-1, pH 4.68, the flow rate was 7.5 BV ·h^-1, the eluting reagent was 80% ethanol (4 times the volume of column) and eluting velocity was 7.5 BV ·h^-1. Conclution : The technological condition will provide bases for the preparation technique of Traditional Chinese Medicine containing ginsenosides.