大黄总蒽醌大孔树脂纯化工艺优化

Optimum Purification Technology of Total Anthraquinones in Radix et Rhizoma Rhei by Macroporous Resin

摘要:

目的: 筛选大黄蒽醌类成分的最佳纯化工艺。 方法: 以总蒽醌类成分的含量为指标,比较不同型号的大孔树脂对大黄蒽醌类成分的吸附性能和解析能力,从而筛选出适宜的树脂类型。采用单因素考察法筛选树脂纯化工艺中的最大上样量、上样速度、洗脱剂浓度、速度、用量等参数。 结果: AB-8型大孔树脂纯化大黄的工艺,固形物收率由原来的38.03%下降到12.23%,主要蒽醌类成分的洗脱率82.96%。 结论: AB-8树脂吸附大黄总蒽醌的纯化方法是切实可行的,同时具有良好的应用前景。

Abstract:

Objective: To screen out the optimum technology for purification of total anthraquinones from Radix et Rhizoma Rhei. Method: Adsorption performance and desorption capacity were compared with different types of macroporous resin with the content of total anthraquinones as the target, so as to select the appropriate resin category. Thus, specific parameters of greatest sample volume, sample speed and elution solution etc were selected from resin purification technology by single-factor study. Result: The technology for AB-8 macroporous resin to purify total anthraquinones from Rheum could not only guarantee the elution rate of 82.96% of major effective indigent, but also decrease the solid content from 38.03% to 12.23% at the same time. Conclusion: AB-8 had a good effect on the adsorption of total anthraquinones from Rheum and has a good appliccation prospect.