猕猴桃根多糖脂质体包封率测定方法研究

Study on Establishing Method to Deternine APPS Liposome Entrapment Efficiency

摘要:

目的: 建立猕猴桃根多糖脂质体包封率的测定方法。 方法: 采用阴离子交换树脂法分离脂质体和游离APPS,采用紫外-可见分光光度法测定游离APPS含量,计算脂质体包封率。 结果: 以纤维素DE-52阴离子交换树脂为填料,采用1.0 mol ·L^-1NaCl溶液洗脱,游离APPS的回收率99.21%,空白脂质体的回收率0.07%;线性范围11.66~58.29 mg · L^-1(r=0.999 4),日内和日间精密度均符合要求(RSD均小于2%),方法平均回收率99.28%,RSD 0.66%;APSP脂质体的平均包封率70.16%。 结论: 此法简便、可行,可用于猕猴桃根多糖脂质体包封率的测定。

Abstract:

Objective: The method was established to determine the entrapment efficiency for the APPS liposome. Method: Anion exchange resin was used to separate the liposome and free APPS; UV-visible spectrophotometry was used to determine the content of APPS, and liposome encapsulation efficiency was calculated. Result: The method established was as follows: the anion-exchange resin was used with 1.0 mol ·L^-1NaCl as eluent; the free APPS recovery was 99.21%, liposome recovery was 0.07% with the linear range of 11.66-58.29 mg·L^-1(r=0.999 4). The intra- and inter-day precisions (RSD) could meet the requirements (within 2.0%); the average recovery was 99.28%, RSD was 0.66%. The encapsulation efficiency of APPS liposome was 70.16% on average. Conclusion: This method is simple and feasible, and can determine the entrapment efficiency for the APPS liposome.