栝蒌根丸水提取工艺正交优选

Study on Extraction Process for Berberine of Kuolougenwan with Orthogonal Design

摘要:

目的: 优选栝蒌根丸水提取的最佳工艺。 方法: 采用4因素3水平正交设计,高效液相色谱法测定小檗碱含量,以提取物中小檗碱含量为检测指标,筛选出栝蒌根丸的优化提取条件。 结果: 栝蒌根丸的最佳水提取工艺为加10倍量水煎煮2.0 h,提取2次。 结论: 栝蒌根丸水提取工艺工艺简单、易于操作、不使用有机溶剂、成本低廉。

Abstract:

Objective: To study the optimized extraction process of berberine hydrochloride, one of the effective parts of Kuolougenwan. Method: An orthogonal experimental design L₉(3⁴) test was adopted in this study. And the content of berberine hydrochloride was determined to fit its quality standard, screening out the best method of extraction conditions. Result: The best extracting technical conditions were as follows: 10 times amount of water, extraction for 2 hour and extracted 2 times. Conclusion: The optimized process is simple, fast and feasible with good stability and repeatability.