多指标正交试验优选升麻提取工艺

Optimization Investigation of Extraction Technology of Rhizome Cimicifugae by Orthogonal Test with Multi-targets

摘要:

目的: 优选升麻的最佳提取工艺。 方法: 首次以升麻总皂苷(以升麻苷H-1计)和总酚酸(以咖啡酸、阿魏酸和异阿魏酸含量之和计)的含量为综合评价指标,采用多指标正交试验法进行提取工艺研究,得升麻的最佳提取工艺。 结果: 升麻的最优提取工艺为6倍量70%乙醇-0.1 mol ·L^-1 HCl(95 ∶5)提取3次,每次2 h。 结论: 优选得到的工艺稳定、可行,为规范升麻的提取工艺提供了理论依据,也为升麻进一步的合理研究与利用奠定基础。

Abstract:

Objective: To optimize the extraction technology of Rhizome Cimicifugae. Method: The content of total saponins (measured by cimicifuga glycosides H-1) and total phenolic acids (the sum of ferulic acid, isoferulic acid and caffeic acid) was selected as the evaluation index for the first time. Orthogonal test was carried out to achieve the best extraction technology of Rhizome Cimicifugae by multiple guidelines grading methods. Result: The optimum extraction condition was that the medicinal materials were extracted 3 times with 6 times amout of 70% alcohol-0.1mol·L^-1 HCl (95 ∶5) and for 1.5 hours per time. Conclusion: The optimum extraction condition was stable and feasible. It will provide a theoretical basis for regulating the extracting process of Rhizome Cimicifugae and lay the foundation to the further research and use of Rhizome Cimicifugae.