龙血竭中黄酮类成分提取工艺研究

Study on Optimum Extraction Process of Total Flavonoids in Resina Draconis

摘要:

目的: 确定龙血竭中黄酮类成分的提取工艺。 方法: 采用单因素考察及正交试验设计,以总黄酮的含量为考察指标优选提取工艺。 结果: 龙血竭中黄酮类成分提取的优选工艺为加药材8倍量的乙酸乙酯提取2次,每次1 h;碱溶精制用提取物30倍量的0.35%NaOH溶液。 结论: 采用优选工艺,龙血竭中总黄酮提取效率高且稳定。

Abstract:

Objective: To optimize the extraction process of total flavonoids in Resina Draconis. Method: The extraction of total flavonoids was chosen as the assessment index. The extraction process was optimized with the orthogonal design using spectrophotometer to determine total flavonoids content at 508 nm wavelength. Result: The optimum ethnol extracting process conditions were as follows adding 8 fold of ethyl acetate, extracted two times, heating for 1 hour. Conclusion: The optimum extraction process of total flavonoids in Resina Draconis was reliable and efficient.