单因素和正交试验结合优化当乌分散片中乌药的提取工艺

Optimization of Extract Technology of Radix Linderae from Dangwu Dispersible Tablet by Single-factor Test and Orthogonal Experiment

摘要:

目的: 探讨当乌分散片中的药材乌药的最佳提取工艺。 方法: 以乌药醚内酯质量分数为考察指标,选择提取方法、提取溶剂、提取次数,用单因素考察方法确定因素水平;再以乙醇体积分数、料液比、提取温度、提取时间为考察因素,以乌药醚内酯质量分数为指标,同时考虑收膏率,用正交试验方法对其最佳工艺进行优化。 结果: 最佳提取工艺:超声提取,90%乙醇,50 ℃的提取温度,提取2次,每次20 min,每次6倍量的提取溶剂。 结论: 采用超声提取法具有提取快速,有效成分提取率高等优点, 此工艺合理可行。

Abstract:

Objective: To optimize the optional extraction method of Radix Linderae from Dangwu Dispersible Tablet. Method: Extracting method, extraction solvent and extraction times were selected to determine the factors and levels by single-factor test method with the content of linderane as index. Then content of linderane was used as index to evaluate the technologies based on orthogonal design, considering ointment rate at the same time, in which 4 factors considered were the concentration of ethanol, solvent consumption, extraction temperature and extracting time. Result: The optimum extraction processes was ultrasonic extraction technique, 50 ℃, adding 6 times amount of 90% ethanol, extracting for two times with 20 min each time. Conclusion: The method has the advantage of speediness, higher extraction rate etc. This process is stable and reliable.