正交试验优选橘红贴膏的乙醇渗漉提取工艺

Study on Optimum Technology of Ethanol Percolation Extraction for Citri Grandis Exocarpium Plaster by Orthogonal Test

摘要:

目的: 优选橘红贴膏的乙醇渗漉提取工艺条件。 方法: 以化橘红中柚皮苷提取量为考察指标,采用高效液相色谱法测定其含量,采用L₉(3⁴)正交试验进行优选。 结果: 优选工艺为: 15倍量80%乙醇,浸润24 h, 渗漉速度5 mL ·min^-1 ·kg^-1。3批验证试验化橘红中柚皮苷平均转移率为79.0%。 结论: 优选的提取工艺合理,转移率较高。

Abstract:

Objective: To optimize the technical conditions of ethanol percolation extraction for Citri Grandis Exocarpium Plaster. Method: The content of naringin extracted from Citri Grandis Exocarpium, which was regarded as the marker of detection, was determined by high performance liquid chromatography and was used to optimize the extraction processing conditions by L₉(3⁴) orthogonal test. Result: The optimum extraction condition was that the concentration of ethanol was 80%, the volume of solvent was 15 times of medicinal materials, the soaking time was 24 hours and the percolating speed was 5 mL ·min^-1 ·kg^-1. The average transferring rate of naringin in 3 batches of samples was 79.0%. Conclusion: The optimum extraction process is reasonable and the transferring rate of naringin is higher.