Objective: Research the method of ZTC1+1 natural clarifying agent for the removal of extraneous materials in Paeonia Lactiflora medicine. Method: Single factor design method was used for investigating ZTC1+1 natural clarifying agent of the relevant factors and the optimum combination of factors and alcohol precipitation methods were compared. Result: The effect of ZTC1+1 natural clarifying agent of impurity was better than alcohol precipitation. Conclusion: Natural clarifying agents with ZTC1+1 for aqueous extract of Chinese medicine is a better way to impurity. The method is convenient, economical and safe with the effect of imparity removal.