毛细管区带电泳法测定黄精和玉竹多糖的含量及其单糖组成

Determination of Polysaccharide and Their Monosaccharide Composition from Polygonatum and Odoratum by CZE

摘要:

目的: 建立毛细管区带电泳(CZE)法测定黄精和玉竹多糖含量的方法,并用CZE法测定其单糖组成,为探讨这两种功效相近中药材的药效物质基础提供参考。 方法: 通过正交试验对黄精和玉竹多糖降解条件进行优化。以1-苯基-3-甲基-5-吡唑啉酮(PMP)为单糖的柱前衍生化试剂,用CZE法测定黄精和玉竹多糖的含量及其单糖组成。同时与采用苯酚-硫酸法测定黄精和玉竹多糖含量的结果进行比较。 结果: 黄精和玉竹多糖均由木糖、阿拉伯糖、葡萄糖、鼠李糖、甘露糖、半乳糖、葡萄糖醛酸、半乳糖醛酸等组成,但组成比例差异显著。 结论: CZE法用于测定黄精和玉竹多糖的含量及单糖组成准确、可靠,结果满意。其单糖组成的异同一定程度上佐证了两者功效的相近和区别,提示两者临床上不能混淆使用。

Abstract:

Objective: The method of determining polysaccharides and their monosaccharides composition by CZE was established to provide a reference for discussing the effective substance of Polygonatum and Odoratum. Method: The degradation conditions of Polygonatum and Odoratum polysaccharides were optimized by orthogonal tests. The degradative monosaccharides, which were derived with 1-phenyl-3-methyl-5-pyrazolone (PMP), were separated and determined by CZE, and the results were compared with those which were determined by phenol-sulfuric acid method. Result: The Polygonatum and Odoratum monosaccharides all included xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid and galacturonic acid. However, the ratio was significant different. Conclusion : The analysis of Polygonatum and Odoratum polysaccharides and the composition of monosaccharide by CZE are accurate and reliable with satisfactory results. The similarities and differences of the effectiveness were evidenced by their monosaccharide composition in some degree, and the results suggested that their clinical application could not be confused.