HPLC测定不同产地禹州漏芦中α-三联噻吩含量

Determination of α-Terthienyl in Radix Echinopsis from Different Places by RP-HPLC

摘要:

目的: 采用RP-HPLC法测定不同产地禹州漏芦中α-三联噻吩含量。 方法: 色谱柱为Hypersil ODS₂C₁₈ (4.6 mm×250 mm,5 μm),流动相甲醇-0.1%醋酸溶液(85 ∶15),流速1.0 mL ·min^-1,柱温25 ℃,检测波长352 nm。 结果: α-三联噻吩在0.491 6~6.145 0 μg峰面积与进样量呈良好线性关系(r=0.999 9),平均加样回收率为98.83%。 结论: 该法简便、快速、准确、专属性强、重现性好,不同产地禹州漏芦中α-三联噻吩含量均符合《中国药典》(2010年版)不得少于0.2%的规定。

Abstract:

Objective: Determination of α-terthienyl in Radix Echinopsis from different places by using RP-HPLC. Method: The separation was carried out at 25 ℃ on a Hypersil ODS₂ C₁₈ column (4.6 mm×250 mm, 5μm) with MeOH 0.1% acetate solution (85 ∶15) as mobile phase. The detective wavelength was at 352 nm with the flow rate of 1.0 mL ·min^-1. Result: The α-terthienyl was linear within the concentration range of 0.4916-6.1450 μg (r=0.999 9). The average recovery was 98.83%. Conclusion: This method is simple and practical with strong specificity and good reproducibility.The content of α-terthienyl is up to the regulation of no less than 0.2% in Chinese Pharmacopoeia (Edition 2010) from different places.