金匮肾气丸醇提物HPLC特征指纹图谱的研究

Study on HPLC Fingerprints of Alcohol Extract of Jinkui Shenqiwan

摘要:

目的: 建立金匮肾气丸醇提物RP-HPLC指纹图谱的分析方法。 方法: 采用低压梯度洗脱的方法进行色谱分离,实验测定多批样品并记录指纹图谱,计算相似度。 结果: 共确定了16个峰为金匮肾气丸的指纹特征峰,自制样品相似度良好,与不同厂家生产的样品相似度有一定的差异。 结论: 该法精密度高,重复性好,可为金匮肾气丸的指纹图谱评价提供依据。

Abstract:

Objective: To establish the chromatographic fingerprints of Jinkui Shenqiwan by RP-HPLC. Method: A gradient mobile system was applied in the experiment. The fingerprints of different samples were compared with similarity evaluation software published by committee codex. Result: Sixteen peaks were identified as the characteristic fingerprints. The samples made by ourselves had good similarity, but there were great differences among different producers. Conclusion: This method shows high precision and good repeatability; it can be used as the method to assess the quanlity of Jin Kui Shenqiwan.