不同地区锁阳药材及饮片中儿茶素的含量测定

Determination of Catechin in Crude Drugs from Various Habitats and Prepared Medical Herbs of Cynomorium songaricum Rupr. from Different Areas by HPLC

摘要:

目的: 采用HPLC法测定不同产地锁阳药材及不同地区锁阳饮片中儿茶素的含量。 方法: 采用Tigerkin C₁₈(4.6 mm×200 mm,5 μm)色谱柱;流动相为0.04 mol ·L^-1枸橼酸溶液- N,N-二甲基甲酰胺-四氢呋喃(52 ∶5 ∶1);检测波长280 nm;流速1.0 mL ·min^-1。 结果: 测定了10个不同产地锁阳药材中儿茶素的含量,其中9个地区锁阳药材中儿茶素的含量高于0.080％,只有甘肃肃南的最少(0.048%);测定了22个不同地区锁阳饮片中儿茶素的含量,其中只有5个地区锁阳饮片中儿茶素的含量高于0.080%,大部分地区锁阳饮片中儿茶素的含量不低于0.040%。 结论: 不同产地锁阳药材和不同地区锁阳饮片中儿茶素的含量不同,饮片中儿茶素的含量低于药材中的含量。

Abstract:

Objective: To determine the content of catechin in crude drugs from various habitats and prepared medical herbs of Cynomorium songaricum from different areas by HPLC. Method: The determination was carried on Tigerkin C₁₈column, 0.04 mol ·L^-1 citric acid-N,N-dimethylformamide-tetrahydrofuran (52 ∶5 ∶1) as mobile phase with a flow rate 1.0 mL ·min^-1 and detection wavelength at 280 nm. Result: Among the crude drugs from 10 various habitats,the content of catechin in crude drugs from 9 various habitats is over 0.080% except from gannan which was 0.048%; Among the prepared medical herbs from 22 regions,the content of catechin in 5 various habitats is over 0.080%, the content in most prepared medical herbs was over 0.040%. Conclusion: The contents of catechin in crude drugs from various habitats and prepared medical herbs of Herbe cynomorii from different areas were different,the content of catechin in prepared medical herbs was lower than that in crude drugs.