亮叶杨桐指纹图谱研究

HPLC Fingerprint Analysis of Adinandra nitida

摘要:

目的: 采用反相高效液相色谱法建立亮叶杨桐叶指纹图谱。 方法: 采用反相高效液相色谱法,以Kromasil C₁₈(4.6 mm×250 mm,5 μm)为分离柱,乙腈-0.1%磷酸溶液梯度洗脱;流速1 mL ·min^-1;检测波长254 nm。 结果: 以梯度洗脱得到的色谱图各色谱峰分离较好,达到指纹图谱的技术要求,10批样品相似度大于0.9。 结论: 采用 HPLC指纹图谱检测技术可有效控制亮叶杨桐叶药材的质量。

Abstract:

Objective: To establish the fingerprint of leaves of Adinandra nitida by RP-HPLC. Method: The chromatographic conditions were as follows: a Kromasil C₁₈ column was used; the mobile phase was composed of acetonitrile-0.1% phosphoric acid with gradient elution; the flow rate was 1 mL ·min^-1 and the UV absorbance detection was set at 254 nm. Result: Under the selected chromatographic conditions, 10 batches of HPLC fingerprint of A. nitida with good similarity were obtained, no less than 0.9. Conclusion: Quality of A. nitida can be controlled effectively by HPLC- UV fingerprint.