HPLC同时测定乳癖消颗粒中7种成分的含量

Simultaneous Determination of Seven Components in Rupixiao Granule by HPLC

摘要:

目的: 建立HPLC同时测定乳癖消颗粒中芍药苷、三七皂苷R₁、人参皂苷Rg₁、连翘苷、哈巴俄苷、人参皂苷Rb₁、去氢木香内酯7种成分的含量。 方法: 采用AlltimaC₁₈色谱柱(4.6 mm× 250 mm, 5 μm);乙腈-水为流动相,梯度洗脱,检测波长203 nm,柱温30 ℃,流速1.0 mL ·min^-1。 结果: 芍药苷、三七皂苷R₁、人参皂苷Rg₁、连翘苷、哈巴俄苷、人参皂苷Rb₁、去氢木香内酯线性范围分别为3.906 25~125 μg (r=0.999),9.375~300 μg (r=0.999),37.5~1 200 μg (r=1.000),2.187 5~70 μg (r=0.999),3.75~120 μg (r=0.999),25~800 μg (r=0.999),0.625~20 μg (r=1.000);平均加样回收率分别为97.95% (RSD 0.71%),99.05% (RSD 1.11%),100.70% (RSD 2.46%),102.74% (RSD 0.35%),103.06% (RSD 0.55%),98.25% (RSD 1.68%),100.16% (RSD 1.82%)。 结论: 本方法方便、稳定、可靠,可用于乳癖消颗粒的质量控制。

Abstract:

Objective: To establish a method for simultaneous determination of seven components such as paeoniflorin, ginsenoside R₁, ginsenoside Rg₁, forsythin, harpagoside, ginsenoside Rb, dehydrocostuslactone contained in Rupixiao granule. Method: HPLC was conducted on an Alltima C₁₈ column (4.6 mm×250 mm, 5 μm) at 30 ℃; the mobile phase was consisted of acetonitrile (A)-water (B) with gradient elution at the flow rate of 1.0 mL ·min^-1 detected at 203 nm. Result: The linear range (mg ·L^-1) of paeoniflorin, ginsenoside R₁, ginsenoside Rg₁, forsythin, harpagoside, ginsenoside Rb, dehydrocostuslactone was 3.906 25-125 (r=0.999), 9.375-300 (r=0.999), 37.5-1 200 (r=1.000), 2.1875-70 (r=0.999), 3.75-120 (r=0.999), 25-800 (r=0.999) and 0.625-20 μg(r=1.000) respectively. The average recoveries were 97.95% (RSD 0.71%), 99.05% (RSD 1.11%), 100.70% (RSD 2.46%), 102.74% (RSD 0.35%), 103.06% (RSD 0.55%), 98.25% (RSD 1.68%) and 100.16% (RSD 1.82%) respectively. Conclusion: The developed method was convenient, reliable and accurate for the quality control of Rupixiao preparation.