灰兜巴粗多糖的提取及含量测定

Extraction and Assaying of Huidouba Polysaccharides

摘要:

目的: 研究灰兜巴粗多糖的提取及含量测定,为临床糖尿病的药物治疗提供新的线索,对开发和研究治疗糖尿病的新物质,特别是从天然资源中找到既能治疗糖尿病又无明显不良反应的药物,具有特别重要的意义。 方法: 采用水提醇沉法从灰兜巴药材中提取灰兜巴粗多糖;采用苯酚-硫酸法显色后用分光光度法测定多糖的总糖含量。 结果: 灰兜巴粗多糖总糖的浓度与吸光度在15~50 mg ·L^-1呈良好的线性关系,回归方程:A=0.0152C+0.019 2,r=0.999 3。采用换算因子f=3.347 3计算,灰兜巴粗多糖总糖的平均质量分数为99.92%。 结论: 该试验采用的苯酚-硫酸法易操作、灵敏度高、稳定性和重现性好,可行。该方法可作为灰兜巴粗多糖的含量测定方法。

Abstract:

Objective: To study on the extraction and assaying of Huidouba polysaccharides, to provide new clues for drug treatment in clinical diabetes, and to develop and research new materials for treating diabetes mellitus, especially those from natural resources that is able to treat diabetes mellitus and no obvious side effects, which is of great significance. Method: The method of decocting with water and depositing with alcohol was adopted to extract polysaccharides from Huidouba. And after phenol-sulfuric acid reaction, the method of absorption spectrometry was used to determine the polysaccharides content of Huidouba. Result: The standard curve was linear in the range of 15-50 mg ·L^-1 with correlation coefficient of r=0.999 3.The linear regression equation was A=0.015 2C+0.019 2.The average content of total sugars of Huidouba polysaccharides was 99.92% calculated using the conversion factor f=3.347 3. Conclusion: The method of phenol-sulfuric acid reaction used in determining the content of polysaccharides is simple in operation with high sensitivity, good stability and reproducibility, which can be used for quantity control of the polysaccharides content of Huidouba.