麻黄附子甘草汤的不同配伍方式对其毒性成分的影响

Influences on Content of Alkaloid in Mahuang Fuzi Gancaotang by Different Compatibility Methods

摘要:

目的: 探讨不同配伍方式对"麻黄附子甘草汤"中毒性成分含量的影响规律,明确产生配伍减毒作用的主要配伍因素。 方法: 按不同组合方式对麻黄、附子和甘草进行配伍,结合酸溶碱沉和萃取方法从配伍后的复方中提取出乌头生物碱,利用紫外光谱对经过酸性染料络合的生物碱进行分析,检测其中总生物碱、酯型生物碱和非酯型生物碱的含量。 结果: 配伍后酯型生物碱含量从46%下降到10%以下,而非酯型生物碱含量从54%上升至89%以上。 结论: 麻黄和甘草对附子有显著且相当的"减毒增效"效果,而且三者同时进行煎煮的减毒效果比其他任何煎煮方式的效果都好,对揭示中药配伍机制有指导意义。

Abstract:

Objective: To analyze the changing regularity of the toxicity of the Mahuang Fuzi Gancaotang through different compatibility methods, and to determine the main factor for reducing its toxicity. Method : Different compatibility methods were used to allocate Ephedre sinica , Aconitum carmichaeli and Glycyrrhiza uralensis . The total alkaloids in all samples were isolated by extraction methods and by different pH value solutions. And ultraviolet spectrometer was used to analyze the content of total alkaloid and ester alkaloid in all compatibility samples. Result : The content of ester alkaloid in compatibility samples was decreased from 46% to 10%, while the content of non-ester alkaloid in compatibility samples was increased from 54% to 89%. Conclusion : E. sinica is similar to G.uralensis in decreasing the toxicity of A.carmichaeli largely and retaining its activity. And the effect of decreasing toxicity is better than other compatibility methods when three kinds of medicines were decocted together at the same time. So, it has important significance to reveal the mechanism of compatibility.