槐叶化学成分研究

Chemical Constituents of Sophora japonica Leaves

摘要:

目的: 研究槐Sophora japonica L.叶的化学成分。 方法: 采用有机溶剂提取,反复硅胶柱色谱和重结晶法进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。 结果: 从槐叶二氯甲烷萃取部分共分离得到了3个黄酮醇、4个异黄酮以及8个其他类成分,分别鉴定为槲皮素(quercetin, 1 ),山柰酚(kaempferol, 2 ),异鼠李素(isorhamnetin, 3 ),染料木素(genistein, 4 ),樱黄素(prunetin, 5 ),大豆黄素(daidzein, 6 ),毛蕊异黄酮(calycosin, 7 ),儿茶酚(pyrocatechol, 8 ),原儿茶酸(protocatechuric acid, 9 ),二十二烷酸二十烷酯(eicosyl behenate, 10 ),二十醇(eicosanol, 11 ),二十二烷酸(behenic acid, 12 ),β-谷甾醇(β-sitosterol, 13 ),豆甾醇(stigmasterol, 14 )和胡萝卜苷(daucosterol, 15 )。 结论: 这些化合物均为首次从槐叶中分离得到,其中化合物 7~10,12,14 为首次从该种植物中分得,这些研究为槐叶及槐资源的综合利用提供了基础。

Abstract:

Objective: To study the chemical constituents in the leaves of Sophora japonica. Method: The constituents were isolated by column chromatography and their structures were elucidated by physico-chemical properties and spectroscopic analysis. Result: Fifteen compounds were isolated and identified as quercetin ( 1 ), kaempferol ( 2 ), isorhamnetin ( 3 ), genistein ( 4 ), prunetin ( 5 ), daidzein ( 6 ), calycosin ( 7 ), pyrocatechol ( 8 ), protocatechuric acid ( 9 ), eicosyl behenate ( 10 ), eicosanol ( 11 ), behenic acid ( 12 ),β-sitosterol ( 13 ), stigmasterol ( 14 ) and daucosterol ( 15 ). Conclusion: All of the constituents are obtained in the leaves of S. japonica; among them, compounds 7-10, 12 and 14 are reported in this species for the first time. The results are helpful for the comprehensive utilization of pagoda tree resources.