木芙蓉药材HPLC指纹图谱研究

Fingerprints of Hibiscus Mutabilis by HPLC

摘要:

目的: 采用高效液相色谱法建立木芙蓉药材的指纹图谱,为其品质控制提供依据。 方法: 采用Waters Xbridge C₁₈( 4.6 mm×250 mm,5 μm)色谱柱;流动相乙腈-1%冰乙酸水溶液梯度洗脱;检测波长370 nm;柱温25 ℃。 结果: 10批各产地的木芙蓉药材指纹图谱峰有10个共有峰,峰面积之和大于总峰面积的90%,与对照品比较共有峰中检出芦丁、金丝桃苷、槲皮苷。 结论: 该方法准确可靠,为木芙蓉药材及其制剂质量控制提供了依据。

Abstract:

Objective: To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Hibiscus Mutabilis. Method: HPLC method was set up by Waters Xbridge C₁₈column (4.6 mm×250 mm, 5 μm) with gradient elution; the mobile phase was comprised of acetonitrile-1% acetic acid solution, the UV detection wavelength was at 370 nm with a flow rate of 1.0 mL ·min^-1, and column temperature was at 25 ℃. Result: There were 10 common peaks in the fingerprints of H.mutabilis based on 10 habitats. Sum of peak areas was larger than 90% of total area. As compared with standard sample, rutin, hyperin and quercitrin were checked out. Conclusion: The method is reliable, accurate and can be used as a quality control method for H.mutabilis.