HPLC测定蒙药三臣丸胆红素含量

Ditermination of Bilirubin in Mongolia Drug Sanchen Pill by HPLC

摘要:

目的: 采用HPLC测定三臣丸胆红素含量。 方法 :色谱柱C₁₈(4.6 mm×200 mm, 5 μm);流动相甲醇-乙腈-1%冰乙酸(88 ∶10 ∶2);流速1 mL ·min^-1;测定波长453 nm。 结果: 胆红素0.0118~0.472 μg成线性关系(r=0.999); 回收率为100.86%;RSD1.86%;三臣丸胆红素平均质量分数4.03 mg ·g^-1。 结论: 采用本法测定三臣丸胆红素的含量,操作简便,准确可靠,可用于制定该药品的质量控制指标之一。

Abstract:

Objective: To develop a HPLC method for bilirubin in mongolia drug sanchen pill. Method: The analysis was carried out on a Kromasil C₁₈(4.6 mm×200 mm, 5 μm)and mobile phase was methanol-acetonitrile-1%glacial acetic acid(88 ∶10 ∶2)with a flow rate of 1 mL ·min^-1 detection wavelenth was at 453 nm. Result: Bilirubin was linar in hte range of 0.0118-0.472 μg (r=0.999),RSD 1.86%. Conclusion: The method is qiuck simple and reproducible for the determination of bilirubin in mongolia drug sanchen pill.