制八角枫HPLC指纹图谱研究

HPLC Fingerprint Spectrum of Radix Alangii Preparata

摘要:

目的: 建立制八角枫高效液相指纹图谱。 方法 :采用高效液相色谱法,色谱柱采用Alltech Apollo C₁₈(4.6 mm×250 mm,5 μm),以乙腈-缓冲液(缓冲液:0.2 g庚烷磺酸钠、3.5 g磷酸二氢钾定容至1000 mL)作为流动相梯度洗脱。 结果: 建立了制八角枫的HPLC指纹图谱,方法学考察符合指纹图谱技术要求。 结论: 该方法稳定、准确,可靠,为制八角枫质控指标的制定提供了科学依据。

Abstract:

Objective: To establish the HPLC fingerprint of the pieces of Radix Alangii Preparata. Method: Using the high performance liquid chromatography,the chromatographic column was Alltech Apollo C₁₈ (4.6 mm × 250 mm, 5 μm) and acetonitrile-buffer (buffer: 0.2 g heptane sulfonate, 3.5 g potassium dihydrogen phosphate volume to 1 000 mL)was selected as mobile phase gradient elution were adopeted. Result :Established HPLC fingerprint of R Alangii Preparata, and the results of methodological studymet the technical requirements for fingerprinting. Conclusion: The method is stable, accurate, and reliable, to provide a scientific basis of quality control standard for the R.Alangii Preparata.