延胡索提取物在大鼠肠外翻实验中的吸收研究

Absorption of Extracts from Corydalis yanhusuo in Rat Everted Gut Scas

摘要:

目的: 利用肠外翻模型研究延胡索提取物不同剂量在大鼠不同肠段的吸收特征。 方法: 采用大鼠肠外翻模型,HPLC检测延胡索提取物中的延胡索甲素(CDL)和延胡索乙素(THP),计算各成分的吸收参数,分析它们在肠道不同部位的吸收特征。 结果: 不同浓度延胡索提取物中CDL和THP在各肠段均为线性吸收,R²均大于0.95,符合零级吸收;CDL和THP的吸收速率常数(Ka)均随延胡索提取物给药剂量的增加而增加(P<0.05),表明其为被动吸收。肠道不同部位的吸收实验表明,十二指肠,空肠,回肠对其均有较好的吸收,结肠吸收较差;且肠道对THP的吸收好于对CDL的吸收。 结论: 不同浓度延胡索提取物CDL和THP在肠道不同部位吸收均符合零级吸收速率,其吸收形式可能为被动吸收。

Abstract:

Objective: Using in vitro everted gut scas to investigate the intestinal absorption of the extracts from Corydalis yanhusuo at different intestinal section and different concentration. Method: Corydaline (CDL) and Tetrahydropalmatine (THP) which are representative compositions of the extracts from Corydalis yanhusuo in everted gut scas were detected by HPLC, and the absorption parameter to describe the character of absorption was calculated. Result: The absorption of CDL and THP was linearity in different intestine and different dose, and the square of coefficient correlation exceed 0.95, which consistent with zero order rate process. The Ka of CDL and THP increased along with the raised dosage of the extracts from Corydalis yanhusuo (P<0.05), indicated the passive absorption. The absorption of CDL or THP in duodenum, jejunum, ileum was quick, but the absorption of CDL or THP in colon absorption was slow. And the absorption of THP was quicker than that of CDL. Conclusion: For different dosage of extracts from Corydalis yanhusuo, the absorption of CDL and THP conforms to the zero order rate process at different sections of intestine, with a pattern of passive absorption.