蒲黄与蒲黄炭对血瘀大鼠血液流变性及凝血时间的影响

Effects of Typhae Pollen and Carbo of Typhae Pollen on Hemorheological Parameters and Clotting Time in Blood-stasis Rats

摘要:

目的 :比较蒲黄及蒲黄炭对血瘀模型大鼠血液流变性及凝血时间的影响。 方法: SD大鼠随机分为6组,分别为正常组,模型组,云南白药组0.27 g ·kg^-1,蒲黄生品水提组1.2 g ·kg^-1,蒲黄炭水提组1.2 g ·kg^-1,蒲黄炭粉组1.2 g ·kg^-1。除正常组外,其余组复制急性血瘀大鼠模型,并比较蒲黄生品、炭品对其血液流变性及凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、凝血酶时间(TT)、纤维蛋白原含量(FIB)的影响。 结果: 蒲黄及蒲黄炭均能明显降低血瘀大鼠全血高切黏度、血沉、血沉方程K、红细胞刚性指数。在凝血时间影响方面,蒲黄炭能明显缩短血瘀大鼠PT;蒲黄及蒲黄炭均能明显缩短血瘀大鼠APTT,降低其FIB,且生品作用强于炭品。 结论: 蒲黄及蒲黄炭均能改善血瘀大鼠异常的血液流变学指标,缩短凝血时间,降低纤维蛋白原含量而表现出一定的化瘀止血功效,蒲黄炭的凝血途径多于蒲黄生品,生品在降低FIB方面强于炭品。

Abstract:

Objective: To compare the effects of Typhae Pollen before and after being carbonized on the hemorheological parameters and clotting time in acute blood-stasis model of rats. Method: The SD rats were divided into control, model, Yunnan White Drug-power 0.27 g ·kg^-1, water extract solution of Typhae Pollen 1.2 g ·kg^-1, carbo water extract solution of Typhae Pollen 1.2 g ·kg^-1, carbo power of Typhae Pollen 1.2 g ·kg^-1. Except the control group, blood stasis models were established in the rest groups. The hemorheological parameters, the prothrombinase time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) were detected in these groups. Result: Typhae Pollen and carbo of Typhae Pollen could decrease the ηb (the viscosity of whole blood) at high shear rate, ESR(erythrocyte sedimentation rate), K of ESR and rigidity of erythrocyte, which increased in model rats. Carbo of Typhae Pollen could shorten PT. Typhae Pollen and carbo of Typhae Pollen could shorten APTT, decrease FIB, and the potency of Typhae Pollen was stronger than the carbo. Conclusion: TyPhaePollen and carbo of Typhae Pollen showed the effect of invigorating blood and hemostasis by ameliotating the abnormal hemorheological parameters, shorting blood coagulation time, decreasing FIB. The effect of Typhae Pollen carbo on blood clotting was stronger than Typhae Pollen, while the effect of Typhae Pollen on reducing FIB was stronger than the carbo.