逍遥散对CUMS模型大鼠行为学及 血浆内单胺类神经递质的影响

Effect of Xiaoyaosan on Chronic Unpredictable Mild Stress Model in Rats

摘要:

目的: 研究逍遥散对慢性温和不可预知应激(CUMS)模型大鼠的行为学及血浆内单胺类神经递质含量的影响。 方法: 以逍遥散和两种阳性药(盐酸氟西汀和文拉法辛)为干预药物,应用慢性温和不可预知应激对大鼠进行为期3周的造模,在造模的同时给予实验药物。3周后停止刺激,但继续给药1周。实验过程中,定期测定大鼠体重、糖水偏爱比,以及穿越格数、直立次数和静止时间等。于第29天处死大鼠,采集血样。采用高效液相色谱-荧光检测法,测定各组大鼠血浆中去甲肾上腺素(NA)及5-羟色胺(5-HT)的含量。 结果: 与空白组比较,模型组大鼠造模3周体重增长缓慢(P<0.01),活动次数明显减少(P<0.01),糖水偏爱比显著降低(P<0.05);而与模型组比较,连续给药4周,逍遥散高剂量组(92.5 g ·kg^-1)、中剂量组(46.3 g ·kg^-1)以及阳性对照组均能明显增加大鼠体重和行为活动量(P<0.01),逆转模型大鼠糖水偏爱百分比的降低(P<0.05)。连续给药4周,逍遥散高、中、低(23.1 g ·kg^-1)剂量组以及文拉法辛组能明显提高模型大鼠血浆NA含量(P<0.05)。 结论: 逍遥散对CUMS抑郁模型大鼠表现出抗抑郁作用,且可能与提高模型大鼠血浆中NA含量有关。

Abstract:

Objective: To study the effect of Xiaoyaosan on changes of behavior and plasma monoamine neurotransmitters on chronic unpredictable mild stress (CUMS) model in rats. Method: The stimulus on CUMS model lasted 3 weeks. At the beginning of stimulus,the rats were treated with the decoction of Xiaoyaosan or two positive drugs (fluoxetine and cultural venlafaxine) for 4 weeks. During the experiment, body weight, the percentage of sucrose preference and data of open-field test (including ambulation numbers, rearing numbers, immobility time) were detected, which was to evaluate the anti-depression action of Xiaoyaosan. In the 4^th week, all rats were decapitated after the last treatment, then blood was immediately isolated for detecting the concentration of NA and 5-HT by HPLC - fluorescence detection. Result: After 3 weeks of CUMS procedure, body weight, number of activities and percentage of sucrose preference of the rats in model group decreased significantly compare with control group (P<0.01 and P<0.05, respectively). Continually administrated for 4 weeks, the body weight, activity times and percentage of sucrose preference in the model group treated with Xiaoyaosan (92.5 g ·kg^-1and 46.3 g ·kg^-1) increased significantly (P<0.01 and P<0.05, respectively) compared with model group. Continuous treatment of 92.5,46.3 g ·kg^-1and 23.1 g ·kg^-1 Xiaoyaosan for 4 weeks could increase the concentration of NA in plasma (P<0.05). Conclusions: It suggested that Xiaoyaosan had the antidepression effect and the anti-depression effect was possibly related to increasing the concentration of NA in plasma.