四物汤及其组成药对对大鼠卵巢颗粒细胞增殖的影响

Effects of Siwu Decoction and Its Drug Pairs on Proliferation of Rat Ovary Granulosa Cell

摘要:

目的: 探讨四物汤及其组成药对对大鼠卵巢颗粒细胞增殖的影响,以期阐明配伍因素对于四物汤干预卵巢功能的作用。 方法: 体外培养大鼠卵巢颗粒细胞,在培养体系中加入终浓度为2,20,200 mg ·L^-1四物汤及其各药对的样品,通过MTT法检测细胞增殖情况,观察其对卵巢颗粒细胞生长的影响。 结果: 四物汤对于卵巢颗粒细胞增殖具有显著促进作用,与溶剂对照组比较上升了36.73%;各药对亦具有显著促进作用,其强弱顺序为:当归-川芎(66.86%)>当归-白芍(58.52%)>白芍-川芎(47.03%)>白芍-熟地黄(41.43%)>当归-熟地黄(23.67%);对归芎药对的进一步研究表明,其水提物中当归-川芎1 ∶2配比,50%醇提物中1 ∶1配比和先水提再醇提物中1 ∶1.5配比药对对大鼠卵巢颗粒细胞增殖的影响较为显著。 结论: 四物汤及其5个药对均有促进卵巢颗粒细胞增殖的作用,其中归芎药对在四物汤调节卵巢功能起着重要作用,并且不同的配伍比例、不同的提取方法对于促进卵巢颗粒细胞增殖具有各自的特点和贡献。

Abstract:

Objective: To investigate the effect of Siwu Decoction and its drug pairs on proliferation of rat ovary granulosa cell, and to probe the effect of combination of drug pairs. Method: Siwu Decoction and its drug pairs were added into the cultured rat ovary granulosa cells in vitro at the concentration of 2,20,200 mg ·L^-1, and proliferation of the cells was measured by MTT. Result: Under the treatment of Siwu Decoction, the proliferation of granulosa cells was significant promoted by 36.73% in compare with the control group. The same effect was also found after treating with 5 drug pairs, and the order of promoting proliferation effects was: Angelicae Sinensis Radix-Chuanxiong Rhizoma (66.86%)>Angelicae Sinensis Radix-Paeoniae Radix Alba(58.52%)>Paeoniae Radix Alba-Chuanxiong Rhizoma(47.03%)>Paeoniae Radix Alba-Rehmanniae Radix Praeparata(41.43%)>Angelicae Sinensis Radix-Rehmanniae Radix Praeparata(23.67%). Water preparation (1 ∶2), ethanol preparation (1 ∶1) and ethanol-water preparation (1 ∶1.5) showed the strongest activity in each group extracted by different method. Conclusion: Siwu Decoction and its drug pairs showed significant promotion effects on rat ovary granulosa cell proliferation. Different pairing ratios of Angelicae Sinensis Radix to Chuanxiong Rhizoma and different extraction procedure contribute to different effects on the proliferation of rat ovary granulosa cell in vitro.