黄芪不同有效部位配伍干预糖尿病模型大鼠 的药效研究

Pharmacodynamic Study on Compatibility of Astragalus Active Ingredients in Diabetes Rats

摘要:

目的: 探讨黄芪不同有效部位配伍对糖尿病大鼠血糖、血清胰岛素及脂联素的影响,筛选有效干预糖尿病的黄芪有效部位。 方法: SD大鼠ip STZ 52 mg ·kg^-1诱导糖尿病模型,分别ig给黄芪及其有效部位的不同配伍,同时设对照组;给药30 d后,以血糖仪测血糖,ELISA法测血清胰岛素、脂联素。 结果: 糖尿病模型大鼠体重减轻,血糖升高,血清胰岛素、脂联素水平下降(P＜0.05);酮苷组大鼠体重增加明显(P＜0.05);黄芪组、酮糖组、酮苷组、酮糖苷组血糖降低(P＜0.05),酮苷组优于糖苷组(P＜0.05);黄芪组升高胰岛素作用显著(P＜0.05);各给药组对血清脂联素均有调节作用(P＜0.05),以黄芪、酮苷、酮糖苷组作用显著(P＜0.05)。 结论: 黄芪及其有效部位的不同配伍调节糖尿病大鼠血糖、血清胰岛素及脂联素水平的作用强度有差异,以黄芪黄酮配伍黄芪皂苷药效较优。

Abstract:

Objective: This study was designed to screen different combination of astragalus active ingredients for blood sugar regulation in diabetes rats, and to find the influences on blood glucose, serum insulin and adiponectin. Method: Diabetes rats were induced by STZ (52 mg ·kg^-1, peritioneal injection ), some of which were administered by different combination of astragalus flavonoids, polysaccharides, and astragalosides for 30 days, the control group was parallelly established. After the treatment, detection of blood glucose and ELISA assay of serum insulin and adiponectin were carried out. Result: Body weight of diabetes rats was dropped, the blood glucose of diabetes was ascended,serum insulin and adiponectin descended(P<0.05).The blood glucose descended in astragalus flavonoid+astragalosides group (body weight gained for the group); astragalus group, astragalus flavonoid+polysaccharides group, astragalus flavonoid+astragalosides group and astragalus flavonoid+polysaccharides+astragalosides group. The best group was astragalus group for ascending serum insulin(P<0.05). The treatment groups showed regulative effect on serum adiponectin, the effect of astragalus group, astragalus flavonoid+astragalosides group and astragalus flavonoid+polysaccharides+astragalosides group were obvious(P<0.05). Conclusion: Astragalus and different combination of its active ingredients could influence the content of blood glucose, serum insulin and adiponectin in diabetes rats, the best result came from astragalus flavonoid+astragalosides group.