抗柯萨奇B病毒性心肌炎胶囊复方的优化及抗应激作用

Optimization of Anti-coxsackie B Complex Capsule for Treating Myocarditis Induced by Coxsackie B Virus and Anti-stress Effect of Capsule

摘要:

目的: 正交试验法优化抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)复方。 方法: 用不含交互作用的"正交t值法"设计,以小鼠缺氧存活时间为指标,筛选出K-CoxB-JN的优化处方,对优选的新组方用抗应激反应(心肌缺血后耐缺氧、负重游泳)实验验证。 结果: 原方中7味药优选出西洋参、黄芪、麦冬、五味子四药作为优化方。异丙肾上腺素致小鼠心肌缺血后常压耐缺氧试验结果显示与正常对照组比较,在缺氧情况下生存时间以模型组最短,差异显著(P<0.01),与模型组比较,原方中、低剂量组及新方组小鼠存活时间均明显延长(P<0.01)。负重游泳试验结果显示对照组小鼠游泳时间最短,K-CoxB-JN原方与优化方低剂量组均能明显延长小鼠游泳时间,有明显的抗疲劳作用。两组试验结果均显示原方和优化方中、低剂量组作用无明显差异。 结论: K-CoxB-JN优化方与原方具有相似的提高小鼠抗应激反应能力的作用,但用药剂量明显减少。

Abstract:

Objective: To optimize anti-coxsackie B complex capsule for treating myocarditis induced by coxsackie B virus with the method of orthogonal test. Method: The optimized prescription of anti-coxsackie B complex capsule was selected according to the survival time of mice after oxygen deficiency with the method of orthogonal t value design. The optimized prescription was verified by anti-stress reaction experiments including tolerance to oxygen deficiency after myocardial ischemia and weight loading swimming. Result: Four of the seven components in the original prescription were selected preferentially to compose the optimized prescription, including Panax quinquefolius, Astragalus mongholicus, Radix Ophiopogonis and Chinese magnoliavine fruit. Both the original prescription and the optimized prescription could remarkably prolong the survival time of oxygen deficient mice and the swimming time of weight loading mice, however, there was no significant difference between the two prescriptions. Conclusion: The optimized prescription had similar effects on elevating the ability of anti-stress action in mice as the original one with less dosage.