酒大黄对动脉粥样硬化兔血脂和NO 及主动脉iNOS表达的影响

Effects of Prepared Rhei Radix et Rhizoma with Wine on Serum Lipid, Nitric Oxide and Expression of Inducible Nitric Oxide Synthase in Aorta of Atherosclerotic Rabbits

摘要:

目的: 探讨酒大黄对实验性动脉粥样硬化(AS)兔血脂、血清一氧化氮(NO)含量及主动脉诱导型一氧化氮合酶(iNOS)表达的影响。 方法: 将健康雄性新西兰兔32只随机分为4组(n=8):空白对照组(N组)、AS模型组(M组)、酒大黄低剂量组(L组)和酒大黄高剂量组(H组),常规高脂饮食建立动脉粥样硬化模型。治疗给药12周,全自动生化分析仪检测血清脂蛋白的表达水平,硝酸还原酶法检测家兔血清NO水平,免疫组织化学法检测主动脉组织iNOS蛋白表达,半定量逆转录聚合酶链反应(RT-PCR)方法检测兔主动脉组织iNOS mRNA的表达。 结果: 12周末与模型组相比, 酒大黄低、高剂量组血清总胆固醇(TC),低密度脂蛋白(LDL),血清NO含量明显下降(P<0.01或P<0.05),高密度脂蛋白(HDL)明显升高(P<0.01), 酒大黄高剂量组甘油三脂(TG)水平显著降低(P<0.01)。主动脉组织iNOS组化染色及iNOS mRNA的表达均明显减弱(P<0.01或P<0.05)。 结论: 酒大黄干预能抑制AS兔主动脉iNOS及其mRNA的过度表达,减少NO的产生,降低血脂,有利于延缓动脉粥样硬化进程。

Abstract:

Objective: To investigate possible mechanisms of the intervention effects of prepared Rhei Radix et Rhizoma with wine on serum lipid, nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in rabbit models of atherosclerosis (AS). Method: Thirty-two healthy male New-Zealand rabbits were randomly divided into four groups (n=8): Normal control group(N),AS model group(M), low dose(L)and high dose(H)prepared Rhei Radix et Rhizoma with wine groups. After 12-week treatment,serum lipid was detected with automatic analysis device. The expressions of serum NO was detected with nitrate reductase method,and protein of iNOS was observed by immunohistochemistry method,iNOS mRNA in aortic tissue were detected by RT-PCR method. Result: At the end of the 12^th week, compared with AS model group,levels of serum TC,LDL,and NO in L and H group were significantly decreased (P<0.01or P<0.05), however the level of serum HDL were increased obviously (P<0.01). The serum levels of TG in high dose group were significantly lower than that in AS model group but no significant difference between the low dose and model group. Conclusion: Prepared Rhei Radix et Rhizoma with wine can reduce the expression of iNOS gene and protein in aorta vessels of experimental atherosclerotic rabbits, reduce the production of NO and levels of serum lipids. and can inhibit the progression of atherosclerosis.